How can I detect IL-1B by Western blot?

Hello Everyone, I know IL-1B is a soluble protein. So how to extract it from the supernatant? Please advice. Thanks!

05 January 2021 7,349 5 View

Is it possible to identify/classify rubber using satellite imagery with machine learning?

As of now, I am trying to explore the potential of using high/very high-resolution satellite imagery with machine learning (ML) to identify or map forest cover (e.g. rubber). As a newbie in this...

17 November 2020 7,187 3 View

Are there any EEG facilities for hire?

I need to run a quick EEG experiment. I don't want to spend time and money to set my own lab. I wonder if there exist EEG labs that can run an experiment for me for a fee?

10 September 2020 5,492 3 View

How do you report undetermined Ct values?

I have knocked down a gene of interest using siRNA and am using qRT-PCR to confirm reduced expression. All samples treated with non-specific siRNA have Ct values around 35, but all samples...

29 July 2020 8,198 3 View

Use of spherical silver?

Where is silver in spherical shape used other than for solar cell electrodes? Most of the silver in electronics seems to be flake. Is this statement accurate?

24 May 2020 7,604 3 View

Insect Population Counting and Studying Traps - recommend a good book or source for info on?

Hi, I'm looking for an entomologist that can recommend a good book on the trapping of insects for study. Thanks

30 January 2020 2,710 2 View

IHC: Monocyte vs Macrophage?

How would you distinguish peripheral monocytes from tissue macrophages with IHC? Specifically, I am interested in looking at rodent brain tissue. Any help is appreciated! Thanks, Dan

07 October 2019 2,683 3 View

Where can I find data on urban land cover in the UK from the 1960s?

I'm modelling insect populations over time (1968 - present) in the UK at over 500 sites. I have detected a decline in sites that are mainly suburban (based on 500m radii using the CEH Land Cover...

02 September 2019 535 3 View

Would (groundwater) dissolved noble gas concentration change after isolation from gas exchange with air?

Dear scientists of noble gas and hydrology, Would equilibrium and/or excess concentrations of noble gases change after isolation from gas exchange with atmosphere? (Non-atmospheric noble gas was...

20 February 2019 255 5 View

Is there a way to control for categorical groups to calculate partial correlations for various measurements?

I have a sample size of 30, representing three different groups (one intervention and two controls). I would like to see if there is an association between my dependent variable and 4 self-report...

18 February 2019 4,619 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View