Can mouse T cells be stained with CCR4, CCR5 and CCR7 for FACS along with FoxP3 using a transcription factor fixation/permeabilation buffer?

03 April 2019 0 4K Report

I have stained mouse T cells for CCR7 many times, but have heard mixed things about the compatibility of this antibody (clone 4B12, BD). I am also wondering about CCR4 (clone 2G12 biolegend) and CCR5 ( C34-3441, BD). I use the Foxp3/transcription factor staining kit from ebioscience for Foxp3 staining.

I also stain for CCR7 at 37 degrees C - is this necessary for CCR4 and CCR5?

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