Dear All,
Can microsomes be obtained from liver tissue without ultracentrifugation for in-vitro drug metabolism?
Thank you.
Hello Cengiz Gokbulut
Yes, you can. Please refer to the protocol mentioned in the research paper attached.
Article Isolation and catalytic competence of different animal liver...
I hope this helps.
Best.
Usually, S9 fractions are obtained by centrifuging liver homogenates at 9,000 x g, which is not classified as "ultra" centrifugation.
Take a look at:
Chapter Preparation and Characterisation of S9 Fractions
Thank you, Malcolm and Daniel, for sharing the related connections.
Kind regards
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