Can microsomes be obtained from liver tissue without ultracentrifugation?

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Malcolm Nobre

Hello Cengiz Gokbulut

Yes, you can. Please refer to the protocol mentioned in the research paper attached.

Article Isolation and catalytic competence of different animal liver...

I hope this helps.

Best.

Daniel Carneiro Moreira

Usually, S9 fractions are obtained by centrifuging liver homogenates at 9,000 x g, which is not classified as "ultra" centrifugation.

Take a look at:

Chapter Preparation and Characterisation of S9 Fractions

Cengiz Gokbulut

Thank you, Malcolm and Daniel, for sharing the related connections.

Kind regards