Can microsomes be obtained from liver tissue without ultracentrifugation?

More Cengiz Gokbulut's questions See All

How to use ImageJ to quantify fibres coming off cell?

Dear All, Currently I am playing around with ImageJ in order to help me with a fluorescence image analysis. So I have cells under two conditions, condition one of which there is a Collagen1A1...

05 August 2020 3,255 2 View

Does a T7 endonuclease assay always work?

Does a T7 endonuclease assay always work? Currently I'm using a plasmid pSpCas9(BB)-2A-Puro (PX459) on HEKs to test my guides. Prior I sequence to check guides have been successfully cloned in...

26 January 2020 5,941 3 View

Establishing hiPSC-KO line using store bought CRISPR-gRNA products? From SCBT for example?

Dear All, I was wondering if anyone has any experience using store bought CRISPR-Cas9-gRNA complexes such as the one listed here;...

18 October 2018 5,419 2 View

How can I setup the Wray1997 Decaying Isotropic Turbulence benchmark case?

As a phD researcher, I am trying to validate my solver through use of decaying isotropic turbulence. I preferred to use the Wray1997 data, which would be a good step towards evaluating my solver's...

17 September 2015 736 2 View

Source codes for 3D human face model generation from images

Can anybody suggest a source code for 3D human face model generating from images? Open source and C++ codes are preferred for quick adaptation. Thanks.

29 May 2012 1,158 13 View

Issues regarding EyeTribe eye trackers and the EyeTribe Software (most recent GitHub release)

EyeTribe eye trackers are now obsolete, following the shutdown of the company. Yet we think that there may be labs that are still using these devices. Recently, in our lab at METU Cognitive...

01 January 1970 8,282 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Malcolm Nobre

Hello Cengiz Gokbulut

Yes, you can. Please refer to the protocol mentioned in the research paper attached.

Article Isolation and catalytic competence of different animal liver...

I hope this helps.

Best.

Daniel Carneiro Moreira

Usually, S9 fractions are obtained by centrifuging liver homogenates at 9,000 x g, which is not classified as "ultra" centrifugation.

Take a look at:

Chapter Preparation and Characterisation of S9 Fractions