Cengiz Gokbulut

6 Questions 27 Answers 0 Followers

Questions related from Cengiz Gokbulut

Why the pressure increases suddenly with every injection and then decreasing in 20 second in HPLC?

The pressure increases suddenly with every injection from 120 to 300 bar and decreases to normal in 20 sec or by switching the valve to mainpass Why? Agilent 1260 series HPLC MP: MeOH:ACM:Water...

12 January 2023 6,046 3 View

Any disc filter suggestion for sterilisation?

Hi, Which kinds of 0.2 or 0.22-micron disc filters are suitable for sterilization of water-based solutions? Also, can they be used for large volumes without the need for vacuum power? Thank you.

16 August 2022 9,060 0 View

Can microsomes be obtained from liver tissue without ultracentrifugation?

Dear All, Can microsomes be obtained from liver tissue without ultracentrifugation for in-vitro drug metabolism? Thank you.

11 November 2021 7,816 3 View

Is the mobile phase best solvent for re-suspension of residue following SPE or liquide-liquide extraction of drugs from plasma for RP-HPLC analysis?

I analyse SSRIs (pKa values of them between 9-10) in human plasma by HPLC following liquide-liquide phase extraction. The residue was resuspended with mobile phase (0.25 ml), water: ACN (42:58)...

23 November 2016 307 6 View

What is (are) the reason(s) for changing of peak shape in RP-HPLC-UV ?

What is (are) the reason(s) for changing of peak shape in RP-HPLC-UV in a sequence with same mobile phase (Na Acetate buffer-pH:5: ACN-10:90 isocrat.), column temperature (40C), flow rate (1 ml/min)?

21 July 2016 7,505 3 View

Is there any chiral column for RP-HPLC which is capable of separation of both enantiomers of most drugs?

There are sorts of chiral column in the market. I need a chiral column for RP-HPLC for separation of enantiomers of some drugs. Should I select the column according to the chemical structure of...

17 September 2014 3,420 15 View