Can lost AFLP bands in Clavibacter michiganensis strains profile be explained by DNA methylation?

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What are the frameworks or methodologies to examine written academic ELF?

What should be the frameworks or methodologies to examine written academic ELF? I want to explore the linguistic features of written ELF in research articles.

23 July 2024 4,800 1 View

How to analyse response rates of a questionnaire?

I have developed a questionnaire. I want to try and analyse question drop-off. There is a clear negative correlation between question number and percentage of respondents who answered that...

07 July 2024 9,435 1 View

Can anybody recognize the virus on potato plants?

Infected potato plant was negative for PVY, PVX, PVM, PVS. What can it be?

19 June 2024 6,739 3 View

Is the preference for more established researchers by reputable academic journals leading to infrequent publishing of Bachelor's degree theses?

I think, for a long time, there has been a tendency for journals to favour work from more established researchers or institutions, thus potentially overlooking quality undergraduate research. This...

16 June 2024 6,801 3 View

How do you generate recursive proofs over time series data?

What are the recommended recursive algorithms to generate proofs of time variant and time invariant properties from a time series data. Approaches like recursive statistical tests, symbolic time...

08 June 2024 4,449 0 View

What is the ratio between the density of the bacterial population during anaerobic and aerobic growth of one species?

What is the relationship between the maximal density of a bacterial population during anaerobic and aerobic growth for a specific species - a facultative anaerobe?

01 June 2024 4,950 4 View

Why do Spanish slugs (Arion vulgaris) prefer to feed on the most poisonous or spicy plants?

Spanish slugs choose to feed on some of the most poisonous and spicy plants. Do they become toxic to potential predators due to this? Are there any publications available on this topic?

28 May 2024 3,929 0 View

How build Forest plot in STATA?

(STATA) Hello everybody. I would like to build a Forest Plot graph that includes all this information from the table. I am unable to build the commands to include this information in the chart....

11 May 2024 6,944 6 View

Does air plasma become optically thick at presssures of about hundred bar?

Is there any experimental or theoretical or theoretical knowledge about the optical depth (may be, for different parts of spectrum) of air plasma at pressures about 100 atmospheres?

19 April 2024 5,539 0 View

Is there any known group of Gymnosporangium sabinae that uses Prynus as the only host plant, both for the telial and the aecial stages?

Gymnosporangium sabinae is a rust fungus known as pear rust. It is a heteroecious plant pathogen with Juniperus sabina (savin juniper) as the main primary (telial) host and Pyrus communis (common...

02 April 2024 1,541 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View