Can K562 cells be cultured in DMEM supplemented with L-glutamine and Sodium Bicarbonate?

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Lorenzo P Menzel Popular answer

I would not. Several vitamins and minerals are missing from DMEM that are in RPMI-1640 and Iscove's Modified Dulbecco's Medium...

Lorenzo P Menzel

Güneş Esendağlı

If you will then culture K562 cells for a long period till you see that they are adapted to DMEM (like no problems in viability and compatible growth as in RPMI).

Garsha Bahrieh

The human leukemia cancer cells (K562) can be grown in the RPMI1640 medium.

We have used the same medium for MCF7 breast cancer cells as well.

Mamatha M Pillai

I think DMEM medium is sufficient for growth.

Tapan Sharma

Thank u friends..:)

Rajgokul K Shanmugam

Hi Tapan Sharma,

How did K562 cells grow in DMEM media??

Hi Rajgokul. I used RPMI1640 with 10%FBS and 1% pen-strep cocktail. Good luck!!