I found a couple of answers to questions regarding Western Blot analysis on this platform saying that the ImageQuant Tools software can detect saturated bands in Western Blot. Is that true? And if so, does anybody have a source for that?
For elemental phase (= terminal solid solution based on one metal element) consisting of one major element, Yeh et al. [1], Murty et al. [2] and Gao et al. [3] proposed a small-negative enthalpy...
21 January 2023 522 4 View
Has anyone else dealt with static electricity on Arabidopsis plates? The static causes the seedlings to root upwards. Passing your finger along the top of the plate causes the upward root to...
01 May 2022 3,175 2 View
Hi, I did some 1H NMR measurements to analyse bacteria-infected urine (yellow graph). I have a clear peak at 1.93 ppm (according to literature: acetic acid). However, I cannot identify the peak...
31 March 2022 2,179 1 View
Is there any publication that has investigated how long it takes for nectar to dry within a cell inside the hive, before it is capped? I am aware that it depends on multiple factors such as the...
31 January 2022 439 2 View
Hello everyone, I'm trying to mesh a structure with complex partitions using mesh controls. When doing it on the user interface I'm first selecting all cells. In the following, I have to accept...
03 February 2021 8,600 1 View
Hey there, I am currently working on the statistics of counted and expected genotypes in mice litter of a mutant mouse model. I have two different mutated genes, both alone and in combination, one...
03 February 2021 6,753 2 View
Our transgenic mice show a really strong phenotype which differs a lot from results of another group working with the same transgene. As our mice are on a pure C57Bl6J background and theirs was on...
24 September 2020 2,060 3 View
I was wondering if anybody could give some tips for B cell culture (primary splenic B cells, MACS purified, 5x10^5/ml + 8ug/ml LPS, + 20ng/ml IL-4, in RPMI, 1-2 days). It seems that I can use...
13 July 2020 6,596 0 View
Dear all, I have the following problem: I want to run a co-citation analysis of a certain set of publications. However, some of them are not part of the Web of Science database. Is there a...
13 December 2019 9,839 7 View
I am trying to transduce primary murine cerebellar granule cells using a lentiviral system. I tried transduction with polybrene, but it seems to be quite toxic for my cells. However, transduction...
10 November 2019 8,691 6 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View