I would like to investigate the ability of an experimental treatment to inhibit T cell proliferation/survival in vitro after stimulation with anti-CD3/CD28. I also want to determine if the treatment is broadly toxic. I realize inhibiting T cell activation in vitro will also cause cell death, which makes it difficult to distinguish broad drug toxicity vs. cells dying after selective inhibition of activation. I was wondering if addition of IL-7 or any other treatments will allow naive T cells that do not become activated in culture to survive for 3 days.
Hi Josh,
I am aware you can maintain T cells in culture for up to 2 weeks or more with Il-7 (10ng/ml at least ); but you must replenish the IL-7 at least every two days to be safe (but you could also push for a 3 day interval). I have maintained the T cells in culture with viability at about 80-90% from 24h post initial culture to the end of the 7 days.
Good Luck,
Lillian
I agree with the previous response: IL-7 will keep your naive T cells alive for some time. Twice a week, when you change medium, you add IL-7 at a concentration of 10ng per ml. However, without irradiated feeder cells I am not sure how long T cells will survive.
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