I inoculated radish seeds with some PGPR, after of 7 days I measured the root and shoot, so, I can use the formule for vigor index, but my question is if formule can be used in radish seed germinated
Yes you can use. Please have look on this paper for the procedure - Abdul-Baki A, Anderson JD. 1973. Vigor determination in Soybean seed by multiple criteria. Crop Sci. 13: 630-633
Seed vigor has evaluated by physiological, biochemical and stress tolerance tests. at present computerized image analysis is possible with less human interference. SVIS system is very sucessful in carrot and radish. It will provide both vigor index and growth index. For more details please follow the attached link: http://dx.doi.org/10.1590/1678-992x-2016-0220. Yes, you can use the formula of both vigor and growth index.