Hello,
I just tan out of these two buffer from the iDeal kit for histones and I have the iDeal kit for TF. So instead of buying a new histones kit could I just use the IE 1 and 2 buffers from the TF kit?
Good day! I am the CEO of an online magazine called Order Of The Pen . We specialize in promoting new writers via our platform on Wix and Partnership with Mailchimp for email marketing,...
18 June 2024 4,117 0 View
its about my masters dissertation
10 June 2024 5,490 7 View
Hi all, I have been attempting to do field EPSP but I am not able to elicit a response. I am using a thin walled electrode for the recording electrode at 1.5mOhm resistance. I have filled the...
10 June 2024 2,418 0 View
What is the best extraction method of sulfonamides group from honey samples? during the testing of the same sample over a period of a time we noticed that concentration of sulfonamides drugs...
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I have generated force field parameters for solvents using AmberTools and adopted force field parameters for a lithium salt from a published paper. How can I effectively combine these two sets of...
03 June 2024 6,891 0 View
I am preparing RNA later following the patented protocol. It is mentioned there and in other recipes as well to use H2SO4 to balance the pH to 5.2. If H2SO4 is not available, can I use HCL instead...
30 May 2024 3,220 0 View
I am using IBM SPSS version 21 as a statistical analysis software. My research is about comparing 2 diferent populations. Let's say it's group A and group B. Each group has variables changing...
15 April 2024 692 13 View
I am working on liquid electrolytes. I generated GAFF parameters by using AmberTools. Now, I am trying to create LT files from Moltemplate. I am discussing my issue here in the hope that someone...
02 April 2024 7,067 0 View
Hi, I aim to conduct MD simulations using LAMMPs. I am trying to convert MOL2 and FRCMOD files into the moltemplate file format (LT). Initially, I generated LT files for my molecules, but I am...
19 March 2024 6,192 0 View
My expressed protein is being collected in the pellet fraction and I have used different strategies for making it soluble but still its coming in the pellet. I have used strategies like...
19 February 2024 5,552 0 View
need to review articles on care giving in dementia and challenges faced
12 August 2024 6,424 1 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...
02 August 2024 6,773 12 View
I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...
30 July 2024 1,412 8 View
Hi, I will run an experiment where reaction time will be the dependent variable, tested in 2 conditions (with the same participants). I want to control for a confounding variable (individual...
20 July 2024 1,943 1 View
What happens in snapTotal- seq? (Single-cell Total-RNA Profiling Unveils Regulatory Hubs of Transcription Factors https://www.nature.com/articles/s41467-024-50291-3) In this protocol, several RT...
18 July 2024 10,010 0 View
One of the papers I read by Toby, where (title of the paper was "R factors in Rietveld analysis: How good is good enough?"), he tells us that to get good chi square value, you must have low Rwp,...
17 July 2024 9,668 4 View
I'm currently working on the implementation of pulses (legumes) in bakery applications. In one case, pulse flours could be added to bread to increase the nutritional value. Pulses (in particular...
17 July 2024 7,293 3 View
Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
10 July 2024 9,165 1 View