I extract DNA from M. tuberculosis chelex and i also check the puruty by the ration OD 260/280 is between 1.8-2.0. I want to know if I can use this directly for the sequencing.
We use chelex 100 resin routinely in our lab for the extraction of DNA of different bacterial species. We use this DNA for target specific PCR and Sanger sequencing with a high reliability.
Once I tried to use DNA extracted by Chelex for library preparation for NGS and it did not work at all.