Anyone used Presenilin 1 [C1C2] Antibody by GeneTex?

Hello, I'm performing WB analysis using the Antibody anti-Presenilin 1 [C1C2] (cat number GTX116016) of the GeneTex company. The datasheet specifies that this antibody recognizes the full-length...

03 August 2020 7,086 1 View

How to treat mammalian diel activity patterns as a continuous variable?

Does anyone have experience in treating/converting mammalian diel activity patterns (diurnal/nocturnal/crepuscular/cathemeral) as a continuous variable rather than a factorial one? I am thinking...

13 May 2020 9,562 2 View

Any suggestions for mouse anti-phospho-ERK1/2 antibodies?

Hello, I am looking for a mouse anti-phospho ERK1/2 to perform immunofluorescence staining on cells (human iPSCs). I have experience with rabbit anti-phospho ERK1/2 #4370 from Cell Signalling...

24 April 2019 8,070 4 View

What's the dosage of Dexmedetomidine that you are using for the Drug Induced Sleep Endoscopy?

18 November 2018 1,703 1 View

DNA Output using QIAGEN Plasmid Miniprep kit?

Hi, I used for the first time the QIAGEN Plasmid Miniprep kit to extract DNA from my plasmids. After DNA extraction I've quantified the DNA concentration and the result is a DNA around 200ng/ul...

01 August 2018 5,123 6 View

RNA integrity number for urine RNA?

Dear colleagues, Do you know the minimum RNA integrity number (RNI) accepted for urine RNA in order to consider the sample for the final analysis? Any reference? Thanks, Ilaria

04 November 2017 1,320 2 View

Why by CRIPR-Cas9 I get only point mutations and how can I induce disruption of my protein of interest?

I performed CRISPR/Cas9 in CHO cells to KO the protein Arrestin3. I transfected cells with a single plasmid containing gRNA - Cas9 - YFP. Then I sorted for YFP and I screened clones by sequencing....

26 January 2017 8,704 2 View

Any suggestions on Alcian blue/nuclear fast red counterstain on paraffin slides?

Hi, I'm going to try an alcian blue/nuclear fast red staining on heart mouse paraffin slides. All the protocols that I've found in internet provide a 30' of incubation in alcian blue. Is this time...

23 April 2015 9,149 9 View

How can I measure the concentration of C60 fullerene (nanoparticles) in tissues?

I have exposed clams in vivo to C60 fullerene for 7 days. And I would like to measure a possible accumulation in gills and digestive gland. If you can help me. Thanks in advance.

17 December 2014 5,369 6 View

Could a transfected promoter be susceptible to epigenetic modifications?

I am trying to understand if the promoter that I want to stably transfect into the cells through the use of a plasmid could be epigenetically modified, as occurred for the endogenous promoter. Thanks.

26 October 2014 3,353 6 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

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17 February 2021 4,217 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How to classify and recognize unseen image class in document sorting?

To sort the scanned document image I have to determine the class of document. But no of classes of image will keep on adding even after training. So at time of inference first I will detect unseen...

16 February 2021 4,501 3 View