Ilaria Scurci

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Questions related from Ilaria Scurci

Can I use T2A for bicistronic expression of marker on plasma membrane?

I need to overexpress a cytosolic protein and to sort the positive cells. I can't label the cytosolic protein with gfp for the sorting so I thought to use the t2A sequence to express at the same...

19 November 2017 4,180 0 View

Why by CRIPR-Cas9 I get only point mutations and how can I induce disruption of my protein of interest?

I performed CRISPR/Cas9 in CHO cells to KO the protein Arrestin3. I transfected cells with a single plasmid containing gRNA - Cas9 - YFP. Then I sorted for YFP and I screened clones by sequencing....

27 January 2017 8,799 2 View