I am looking for polymers/biopolymers with high hydrophobicity and high gas permeability. Can any one recommend any types of polymers/biopolymers?

I am looking for polymers/biopolymers with high hydrophobicity and high gas permeability. Can any one recommend any types of polymers/biopolymers?

25 November 2020 5,887 3 View

How to use Incremental/Online learning in face recognition?

I am trying to implement incremental/online learning for a facial recognition application. I've trained a model on a dataset and it works perfectly fine, however, I need to capture new...

23 September 2020 3,819 5 View

How to Fill Up volume in CST MWS?

How to Fill Up volume in CST MWS Hello everybody, Does Anyone know how to fill up a generic volume with a certain material using CST Microwave Studio Software? How to connect a curved plate to...

07 February 2020 4,427 1 View

How to use a CNN model saved as .mat file in python?

Hello everyone, I am going to use a FER2013 fine-tuned pretrained VGG-Face model to extract some features from facial images. Actually, I have the weights and layers of this model saved as a mat...

21 January 2019 4,387 2 View

Which CD20 expressing cell line is better?

Hi there , we are working on antibodies against CD20 antigen ( tositumomab, ofatumumab, Obinutuzumab ) and need a cell line which overexpressing CD20 . as far as I am concerned, Daudi , Ramos...

07 November 2018 1,015 3 View

PCR troubleshooting.what should I do to solve the problem ?

29 August 2018 1,651 6 View

Any suggestion or comment in regard of PCR troubleshooting ?

20 August 2018 150 10 View

Hi.How much blood is needed to gain enough lymphocytes to do RNA extraction?

How many PBMC can be isolated from 10 ml of blood from a healthy donor?

19 July 2018 7,153 3 View

Why does my Blue native gel doesn't run completely?

I have been running blue native gels for my chloroplast samples, the problem is that the gel won't run completely. I have seen gel pictures in different papers that the whole sample runs really...

15 January 2018 4,291 2 View

Is anyone familiar with methods to generate surface waves using COMSOL?

methods to generate surface waves using COMSOL

22 February 2016 5,385 9 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View