I have rotor gene sybr green for the Qiagen equipment, but it´s broken. I want to know if I can use this kit to perfome the qRT-PCR reations on Step One equipment, using 96 well plates.
Yes, provided the StepOne model has a SYBR green channel it will be compatible. If it has any, make sure it meets the sample requirements (i.e. 5, 10 uL sample or passive referene dye) for that instrument.