Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

Can we mix elemental powders of 325 mesh using an agate mortar and pestle?

Elemental Powders like Titanium, vanadium, tungsten, zirconium, hafnium.

04 January 2021 7,357 1 View

How to run spin polarized calculations in quantum espresso?

I would like to run spin polarized calculation using quantum espresso. Is it important to add spin polarization in calculating the wavefunction cutoff, K point convergence and geometrical...

17 November 2020 7,604 2 View

MAX phase synthesis?

We are having titanium, aluminium and graphite powders. But we don't have ball milling facility in our lab. Can we mix these manually using mortar and pestle for obtaining MAX phase powders? How...

16 November 2020 3,436 4 View

Material simulation of Concrete damage plasticity model(CDP), In abaqus?

09 November 2020 268 2 View

How to respond to the comments from multiple reviewers?

I have received comments from three reviewers. many of the comments are similar. do i have to respond to the comments of all the three reviewers individually or can i write a consolidated...

15 October 2020 9,220 4 View

Testing endogeneity in the model through VAR ?

I have the panel data regression model for which have to test the endogeneity. i read one research paper which was using Vector Autoregression (VAR) to test the endogeneity. in that paper, it...

03 October 2020 877 3 View

Enzyme assays of mutant GBA cell lines ?

How do i measure the enzyme activity of human GBA in cos 7 cells that have been transfected with mutant plamids. After 24 hours of plating cells in a 12 well plate, I transfect cos 7 cells with...

30 August 2020 4,496 3 View

How to describe mixed occupancies in the Quantum espresso input file?

I am having trouble describing partial occupancy in the input file. One of the site is occupied by two atoms with (50% occupancy). when I generate input file, the other atom is getting ignored....

19 April 2020 9,383 3 View

Antimicrobial properties of metal oxide nanoparticles?

08 April 2020 3,013 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Which is the most efficient etching solution for nickel based alloys?

Which solution reveals the microstructure and features of nickel-based alloys?

02 March 2021 2,221 3 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

No title

17 February 2021 4,217 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How can I spin a thick layer of organic polymer?

I need to spin a 120um thick film. My current procedure consists of spinning the polymer (about 20um), uv exposure, plasma etch, and then spinning another 20um thick layer, uv again, plasma etch...

10 February 2021 2,055 6 View