Can I use plasma of the infected patient for doing Antibody dependent Cellular Cytotoxicity ?
Hello Deepak Vats
In ADCC, upon activation, NK cells mediate a variety of effector functions, including the release of cytokines and chemokines, and degranulation to kill the neighboring virus-infected cells. Plasma will contain not only the antibody of interest but also variable levels of biologically active cytokines. Chronic infection perturbs cytokine levels which could influence the effectiveness of ADCC response.
NK cells are exquisitely sensitive to exogeneous cytokines. If NK cells are exposed to exogeneous cytokines present in plasma prior to activation, it would differentially alter the effector functions mediated by activated NK cells.
So, activation of NK cells namely, expression of cytokines and/or degranulation by ADCC is likely to be influenced by other factors present in plasma. You need to consider this when you would use plasma of the infected patient for doing Antibody dependent Cellular Cytotoxicity.
Best.
Thanks a lot for the answer @MalcolmNobre.
So, I need to purify specific antibodies against the antigen.
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