I have no clue about world of bacteriophage.
Are there any recipe of other buffer for phage enrichment?
Gelatin is not available in my lab and the work is urgent. It's about isolation of bacteriophage from food. I try google and youtube but I need more detail that this. I will appreciate if any source/textbook or website about basic of phage isolation are suggested.
Thank you in advance
Chooseel
You can use both buffers; however some phages enrich better in Phage buffer (Tris/NaCl/MgCl2/CaCl2) while some phages prefer SM buffer (Tris/NaCl/MgSO4/gelatin). Once you have your phage or phages, you will need to test them to see which buffer enriches better if you are trying to really optimize enrichment.
Good answer Gregory. Gelatine increases stability of isolated phages often. It's not expensive and the normal household powder from food stores may help. It is sufficiently pure for that purpose.
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