Chooseel Bunsuwansakul

10 Questions 3 Answers 0 Followers

Questions related from Chooseel Bunsuwansakul

What is alternative Sterile Catch-All™ Sample Collection Swabs (Epicentre Biotechnologies, Madison WI) for skin microbiome project?

It seem like I can not find one here in Thailand. I just want to know what is the material or technology that they use like is it flocked swab? or foam-tip? is it nylon or other polyester. note...

21 August 2020 3,919 0 View

Alternative PCR tube rack?

Any ideas about alternative PCR rack. There are more than 5000 tubes right now. I don't know why PCR rack is so expensive (for me). I tried polystyrene foam and pipette tip boxes. It doesn't work...

06 April 2020 1,002 3 View

Which brand of Agarorose gel electrophoresis system is worth?

Normal buffer tank and power supply is fine. No need expensive version but practicable design . and the price is second priority. Thank you in advance

15 January 2020 2,453 5 View

Can I use phage buffer in stead of SM buffer in step of phage enrichment?

I have no clue about world of bacteriophage. Are there any recipe of other buffer for phage enrichment? Gelatin is not available in my lab and the work is urgent. It's about isolation of...

03 December 2017 521 4 View

What is the easy and cheap way to check size of total rna after total rna extraction for RNA-seq.

Thank you in advance

31 August 2016 3,867 3 View

What happens if I use Tophat instead of Bowtie software for mapping bacterial transcriptome?

Research paper (p.564, 2nd paragraph: http://www.nature.com/nprot/journal/v7/n3/full/nprot.2012.016.html) told me that Bowtie will align RNA segment with reference genome. TopHat too but TopHat...

11 January 2015 1,131 3 View

How can I be sure that the number of fragments in the cDNA library will be assembled into true copy numbers of biological units such as a gene?

we fragmented mRNA for making cDNA library for example, 2 transcripts are cut into 6 fragments before changed into cDNA. How do I know that these 6 fragments will be from 2 transcripts. If we lost...

06 January 2015 3,055 5 View

Is the reagent such as RNAprotect (Qiagen) really essential for bacteria?

Many bacterial RNA-seq research papers use it before RNA extraction. Is it critical that cells are harvested and then extracted immediately? Thank you in advance. (Newbie in RNA-seq.)

06 January 2015 8,720 1 View

How do I know which hour to harvest bacterial cell for RNA extraction after exposed to antibiotic?

It's about transcriptomics. mid-log phase bacteria divided into 2 conditions, with and without antibiotic but I wanna know the incubation time before RNA extraction some paper said 30min, some...

09 December 2014 3,772 4 View

What is the best McFarland standard machine?

or any machine that include McFarland stand. My lab wants to buy new one. Or anyone wanna donate? lol My field of research is about growth curve and drug susceptibility. The ancient Biomerieux...

14 November 2014 9,085 2 View