How to overcome the tension or frustration with others who are less organized or careless than you are?

Working with others usually good and build a massive amount of experiences, but sometimes there are some drawbacks of it. Careless, less organized, randomness, and commitment are some important...

30 October 2020 2,520 8 View

Tomato pollen internal membrane dye?

Hello everyone, I wonder if you can help to suggest any internal membrane dye that would enable me to stain the generative cell in tomato? unfortunately I have no marker tagged to the cell but I...

28 October 2020 2,335 3 View

Transient heat transfer through 2 walls and to air?

Dear All I am trying to simulate a problem that simplified as shown in the attached file. It has two different walls one inside the other and surrounded by air. I want to simulate the problem in...

14 October 2020 1,002 19 View

How many volume for media that we need to isolate exosomes from media?

Because i isolate 5 ml of culture media without serum and i got 1.2 ng miRNA . And i need 70 ng minimum that we can do PCR

28 September 2020 5,887 4 View

Is there any research points link Statistics and Big Data analytics that can be good points for research in PhD ?

Statistics and Big Data Analytics

02 August 2020 2,830 6 View

Which companies provide Molecular imprinted polymers ( MIPs) ?

19 July 2020 8,876 9 View

What are the differences between Renewable Energy and Energy Efficiency Technologies?

I found that energy efficiency deals with combustion efficiency, using biomass fuels and stuff like that. Can we link between wind energy and energy efficiency by some means?

07 June 2019 2,892 6 View

What are the best mesh quality criterion for inspection element quality?

I know about mesh density, skewness, wrap angles, and aspect ratio. Per literature, aspect ratio is a quick tool to define quality of mesh. Is there a rule of thumb for acceptable values of...

07 February 2019 7,145 4 View

Is Fmincon solver suitable for nonlinear ratio (I.e. NLP/NLP)?

I use fmincon to minimise weight to stiffness ratio. This ratio is Non linear. I tested this solver by using different initial points Xo. However, results are changeable and dependent on Xo. I am...

01 February 2019 3,069 3 View

What are the main considerations in injection mold designing and calculations?

I'd like to know the main parameters that should be considered in designing of injection mold. Also, I need to know a source for standardized calculations.

10 February 2018 6,412 3 View

Are these moths Spodoptera frugiperda?

The caterpillars of the moths were collected as fruitborers in pepper at a farmer. I reared them till the adults hatched and compared them with pictures and descriptions on the internet. Are these...

24 February 2021 7,785 5 View

Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

Detection of insect eggs in stored and processed products?

Currently, in order to identify insect eggs in stored or processed products (such as contaminated flour or wafer samples), staining was performed based on the method mentioned in the below...

15 January 2021 6,362 5 View

Which test to use check normality for count data if I have 3 treatments and one dependent variable?

I am working on a experimental design. I have three treatments(three temperatures) . I have 18 fish under each sample and after a desired time I will check if there is any variation in their...

01 January 2021 9,167 3 View

CRISPR/Cas9 oligo design ideas and suggestions?

I need to design new oligonucleotides that can be used for gRNa/Cas9. The oligonucleotides need to target a gene from melangoster drosophila. Where do I start? How to find the gene to knock...

15 December 2020 4,729 3 View

What is the preferable incubation period in injecting ethanol into the duck eggs for antiteratogenic studies?

I have been working on the anti-teratogenic activity of a plant extract towards ethanol induced duck embryos however, many studies use the 14 days incubation period and then remove the embryo for...

17 November 2020 4,701 5 View

How can I get eggs of an univoltine casebearer moth for a threshold study?

I am planning to conduct a cage study to determine a critical density of an univoltine casebearer moth in red clover seed crop. This requires to release first instar larvae in the cages with the...

02 November 2020 6,577 3 View

How can I get help to identify a species of Coniopterygidae from Peru?

I found a species of Coniopterygidae preying on eggs and nymphs of Aleurothrixus floccosus in a lemon tree. I have followed its biology and have pictures of eggs, larvae, pupae and adults. With my...

16 October 2020 6,421 2 View

What is the protocol of eggs sampling from an eggs production unit ?

Dear researchers greetings, I'm working on eggs quality for my Ph.D thesis and I want to know what is the protocol for eggs sampling from a production unit. The main questions I have are the...

13 October 2020 9,716 7 View