You certainly can. Lentiviral vectors are expresion vector and will behave at such in transfection.

If you want the effect to be transient, you have to make sure you use non-integrating lentiviral vectors.

Hi Suipwksiow,

Transfection is not necessary transient and transduction not necessary stable as you seems to mean in your question. Transduction is when you do gene transfer with viral vectors and transfection is when you do that with plasmids, it is just to clarify because many people does'nt well used these terms.

As Julie said, to do transient transduction you can used non integrating lentiviral vectors, you can find different versions (D64V, LQ, N etc...) which are not necessary equivalent in terms of efficency and integration background, the choice will be done according your experimental context and needs.

Recently, have emerged a new class of LV: RNA lentiviral vectors which are transient and without any background of integration. It is like RNA transfection but with the advantages of transduction!

https://patents.google.com/patent/US20140286907A1/no

Let me know if you need further information, it is my lab which has developed RNA LVs ;)

Regards

Niolas

There is a lenti focused webinar coming up that might address your concern and help answer any additional questions.

Title: Knockdown, Knockout, Validate: Lentivirus delivers payload in vitro and in vivo Date: Wednesday, May 16, 2018 Duration: 60 minutes Times: 10:00 AM (CST) Speaker: @Christy Hoffmann

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