I ran two RNA Pico chips, one after the other. I used the RNA ladder from the same exact aliquot, but for one chip it appears to only contain the upper marker; which still gives me the RIN numbers I need, but I would also like a proper concentration estimate. I would strongly prefer not re-do the chip to avoid an extra freeze-thaw cycle of the RNA.

Could I use the ladder file from my second chip to recalculate the standard curve for the first one? Or somehow edit the XAD file with the ladder data from the second chip so the fragment sizes and concentrations are recalculated?