Since research grade collagen and gelatin are quite expensive and i have to import which means it will take much time i am searching for alternatives. Can collagen hydrolysate or gelatin hydrolysate be alternatives to collagen and gelatin?
Thank you in advance..
You probably can but what would be the point? Food grade is not tissue culture grade, if you're aiming to use this with cells. Food grade quality is based on other criteria and quality then what cells actually need. It's probably also less clean or conatins unwanted impurities (endotoxins etc.) You might be optimizing something which is not translatable in the end.
In addition to the points in the previous answer, the range of molecular weights will be much broader in food grade material. Can you extract and purify collagen in your lab? For example, from rat tail tendons or from cartlage?
If you have no economic possibility or laboratory means to obtain collagen from animal tendon. Before using food grade collagen you should use the technique of spectrophotometry to quantify the present collagen and also analyze its degree of purity by polyacrylamide gel electrophoresis with SDS, based on the method of Laemmli.
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