I want to do cell viability assay using FACS with Doxorubicin treated cells. Since Dox and PI absorbance wavelength is very close, I am wondering if there will be a cross talk between them?
Hi Ramya, yes they will interfere with each other. We had an issue with this on FACS. It would be best to move your viability dye down the spectrum to perhaps 7AAD.