Hello Nur.

Yes, you could use if you add 1mg BSA/ml DMEM as a carrier protein and a preventing bFGF from losing activity by attaching to the container walls. Thus, make 5ul aliquot tubes (1,000~2,000X stock solution) and store at -80C until use. Do not re-store at 4 or -20 or -80C.

However, some of DMEM components are degrading as time goes. Therefore, it is best to make 5ul bFGF in PBS containing 1mg BSA/ml (PBS + BSA). It will goes forever at -80C without deterioration.

Make PBS + BSA, millipore (0.22 um) and dilute bFGF with the millipored PBS + BSA to make 1,000~2,000X stock solution to be added to the cell culture.

Best wishes.

Hi Dr. Sang,

Thank you for your reply. Is it ok if I use FBS? as FBS also composed of BSA and other proteins.

Hi Nur.

It depends on the properties of the experiments. If you are not using serum for cell culture, then you should not use FBS even if it is extremely low.

If you are culturing cell in the presence of serum plus bFGF, then it would be OK. Heat-inactivation before mix and milliporing are needed after PBS + FBS mix.

Regards.

Dear Dr. Sang,

Thank you so much for your reply. It helps me a lot.

Regards,

Afiqah