I am currently working with RAW cell lines. For passaging I am using DMEM medium w/o sodium pyruvate. Will it cause any problem during passaging? What is the importance of having sodium pyruvate in DMEM medium for RAW cells?
I only add glutamate, heat inactivated FCS, antibiotic/mycotic mix to my DMEM for culturing Raw264.7 cells. Been culturing these cells for more than a year. Pyruvate is just an extra energy source.
I only add glutamate, heat inactivated FCS, antibiotic/mycotic mix to my DMEM for culturing Raw264.7 cells. Been culturing these cells for more than a year. Pyruvate is just an extra energy source.