What is the nutrient requirements for E.coli BL21 CodonPlu RIPL strain growing in M9 medium?

Hello. I want to isotopically label the my protein, and I express it in BL21 RIPL using M9 minimal media. It grows extremely slow. I inoculate the colony from a fresh M9 plate into 5ml M9 medium...

17 February 2021 2,852 2 View

Do you have experience of second round PCR amplification issue?

When 1st-round PCR amplification was finished , I could get a clear expected band, but the amount was very low, so I cut the band and run second-round PCR with same condition, it didn't work, I...

21 January 2021 888 4 View

Can someone recommend me a classic book of liquid crystals?

I am currently into liquid crystals and have no related background. Can someone tell me which book is the most instructive one to learn basics of liquid crystals? I am more interested in different...

22 November 2020 4,362 6 View

How does the influence of metal ions on the fluorescence intensity of a compound?

We found an interesting phenomenon that metal ions could not influence the fluorescence of a compound when the compound was dispersed in solvent. However, the fluorescence of the compound was...

31 October 2020 6,568 3 View

Is K-silicate gel a porous material?

I coated a stone surface with K-siilcate. Apparently, it does noe seal the surface since the water penetrae the stone easily and the absorption coefficient does not change too much. However, the...

05 October 2020 3,435 5 View

Fluent how to draw a graph of coordinate changes over time?

When simulating waves, it is necessary to detect the coordinate change at a certain point of the wave surface, but there is no in fluent.

04 October 2020 1,541 1 View

How to quickly know the new research area landscape and find a research gap in this new area?

As a Ph.D. candidate who enters a new research area that differs from the research topic in the master period (e.g. shift form crop breeding to soil ecology), how to quickly know the new research...

23 September 2020 8,131 5 View

How to build epoxy model in GAFF for Lammps?

Dear all, I am a beginner in Molecular Dynamics. I build a pre-reaction epoxy model using Materials Studio and plan to perform the cross-linking procedure using Lammps. However, the msi2lmp tool...

26 August 2020 7,697 1 View

How to detect brown pigments generated in red wine aging process ?

During aging or storage, the concentration of monomeric anthocyanins decreases rapidly, while low level of polymeric anthocyanins generates, and the color of wine turns brick-red gradually. In...

17 July 2020 6,032 4 View

How to define the particle agglomeration in composite materials or foundry alloys and measure its size distribution quantitatively?

28 June 2020 7,614 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View

Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View

Can you correct the wt%/at% of an incorrectly assigned peak in EDS?

For instance, I get a spectrum and the software incorrectly assigns a Si peak as W. If I don't catch it on the software and only have the report (which lists both wt% and at%), is there a way to...

10 February 2021 7,665 4 View

How to model Type 1 collagen structure for finite element analysis ?

I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...

07 February 2021 5,487 3 View

In your view, which current SEM is most recommended for EBSD/TKD and EDS analysis?

I know that this is a very difficult question, as the areas of application can vary greatly. However, if you want to operate low as well as high resolution (from a few millimeters to nanometers),...

02 February 2021 4,985 4 View

Solubility of PMP in solvent?

What is the maximum solubility limit of poly(4-methyl-1-pentene) in N,N, dimethylformamide? I gone through solubility parameter concept but I did not get the answer for maximum amount of polymer...

02 February 2021 947 3 View

Dear Colleagues, I am looking for a way to dop of electrospining nanofibers on the HEPA filter.Someone can guide me?

Electrospining method is important for production of nanofiber .If doped with nanoparticles ,can be used for remove of contaminant such as VOC , but don't have mechanical strengh . For finding a...

24 January 2021 7,290 3 View