Yes, primers that work for qPCR can also be used in conventional PCR reactions. Just keep the same thermal cycling parameters.
Conventional PCR usually have bigger amplicons than qPCR. If you have a qPCR with a small amplicom like 65-85 bp, it may be difficult to visualize in certains eletrocphoresis.
Be careful because degenerated primers usually do not have good qPCR eficiency.
if the primers works well in qPCR that will also work in general PCR. Remember that the amplification is same with both the PCR the only advantage with qPCR is that you can quantify the targets
Hi Kamal,
Yes, it is in principle. See the link attached.
https://www.researchgate.net/post/Can_the_primer_designed_for_qPCR_RT_PCR_be_used_for_conventional_PCR_too
Hi everybody
Thank you so much for your good guidance.
These are my primers. but I'll try to use them by Thermocycler PCR...
F(GCCGATMGVAGCAAACTSAC)
r(STGATVCKTTTNCGGTCCCA)
F(AGYATGCTGATTGCGGGNC)
r(TGTAVTKTTTSCCGATCCAG)
That's kind of you.
Thank you all
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