Can anyone help us with our Granny smith disease or pest?

As you see apple picture today I found this problem with granny smith variety of apples. I saw something like insect's sting with halo around that!!! I don not know what it would be. Please write...

04 May 2018 4,817 8 View

Erwinia amylovora selective media

which one of mediums could be useful for detective of Erwinia amylovora as a selective? Thanks

06 July 2017 4,898 3 View

Which one of fungicides can be useful instead of PCNB for PARPH medium?

Which one of fungicides(Toxin) can be useful instead of PCNB for prepare PARPH medium? Because unfortunately I couldn't find PCNB for Phytophthora medium!!! We have some problem in apple MM9 by...

01 February 2017 7,605 7 View

How the glycerol can increase the ability of ice nucleation activity in these bacteria(ina bacteria)?

01 February 2016 2,139 0 View

What is the difference between HR(hypersensitivity reaction) in bacteria and fungus?

Bacteria have hrp protein and (TTss) but fungi doesn't have this system. and how they active this way and by what mechanism ? What is difference between their symptoms and their reaction in plants?

01 February 2016 7,881 1 View

Which bacterial outgroup?

10 November 2015 2,864 3 View

Is there any method for metallothionein detection in fungi except spectrophotometry and hplc?

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10 November 2015 9,864 0 View

How can I find metallothinein origin in genome of Trichoderma to design Primer?

this oringin isn't detected in Trichoderma spp. . Or I don't know ...

09 October 2015 2,466 2 View

Can I use degenerate primers that aligned to real time PCR and used with that, by Thermocycler PCR?

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07 August 2015 5,969 5 View

I have a pair of degenerate primers so I want to try it on thermocycler PCR (not Real time PCR). Do you think that it can be multiplied?

TM of primers 58.4 and 56.4 I don't know anything about annealing temperature, please guide me to find appropriate annealing temperature. Thanks.

07 August 2015 579 13 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View