Can anyone help us with our Granny smith disease or pest?

As you see apple picture today I found this problem with granny smith variety of apples. I saw something like insect's sting with halo around that!!! I don not know what it would be. Please write...

04 May 2018 4,817 8 View

Erwinia amylovora selective media

which one of mediums could be useful for detective of Erwinia amylovora as a selective? Thanks

06 July 2017 4,898 3 View

Which one of fungicides can be useful instead of PCNB for PARPH medium?

Which one of fungicides(Toxin) can be useful instead of PCNB for prepare PARPH medium? Because unfortunately I couldn't find PCNB for Phytophthora medium!!! We have some problem in apple MM9 by...

01 February 2017 7,605 7 View

What is the difference between HR(hypersensitivity reaction) in bacteria and fungus?

Bacteria have hrp protein and (TTss) but fungi doesn't have this system. and how they active this way and by what mechanism ? What is difference between their symptoms and their reaction in plants?

01 February 2016 7,881 1 View

How can I find the region of gene in complete genome of bacteria?

I want to find ice nucleation activity gene in genome of Pseudomonas, Xanthomonas, Erwinia etc. by software or Websites such as NCBI. Guide me please. Thank you.

11 December 2015 3,503 3 View

Which bacterial outgroup?

10 November 2015 2,864 3 View

Is there any method for metallothionein detection in fungi except spectrophotometry and hplc?

.

10 November 2015 9,864 0 View

How can I find metallothinein origin in genome of Trichoderma to design Primer?

this oringin isn't detected in Trichoderma spp. . Or I don't know ...

09 October 2015 2,466 2 View

Can I use degenerate primers that aligned to real time PCR and used with that, by Thermocycler PCR?

.

07 August 2015 5,969 5 View

I have a pair of degenerate primers so I want to try it on thermocycler PCR (not Real time PCR). Do you think that it can be multiplied?

TM of primers 58.4 and 56.4 I don't know anything about annealing temperature, please guide me to find appropriate annealing temperature. Thanks.

07 August 2015 579 13 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View