Can i use coomassie brilliant blue R-250 in Bradford assay?

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Andrew Kirkham

Yes, but the results will be less certain. The best results are always obtained from a commercial kit.

Alemu Tekewe Mogus

I agree with Andrew. Use the commercial kit for obtaining reliable results.

Adam B Shapiro

The Bradford reagent uses Coomassie Blue G, not R.

Coomassie Blue R is for staining protein gels (although Coomassie Blue G can be used for protein gel staining as colloidal Coomassie Blue).

Amira Sabry

Thanks a lot for all the replies

Amina Boudouaya

I'm so interested in knowing the answer too