Can i use chemically competent e.coli cells bought from Promega as starting material to again make them competent by multiplying on SOC/LB medium?

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What is Differential-Fed in microstrip patch ant? How it is design in CST studio? What is process and procedure to do Differential-Fed in CST studio?

What is Differential-Fed in microstrip patch antenna ? How it is design in CST studio? What is simulation process and procedure to do Differential-Fed in CST studio ?

08 July 2024 7,464 1 View

How to know the TM/TE modes in antenna design in CST studio?

How to know the TM/TE modes in antenna design in CST studio? Example: TM01 ,TM02 ,TM10, TM12, TM30 like this... how can identify the antenna is operate the above said mode? can any one explain...

08 July 2024 6,609 2 View

What is co-polarization and cross polarization? How can plot it from CST studio?

what is co-polarization and cross polarization? How can plot it from CST studio? what are steps and process for find out co-polarization and cross polarization from CST studio?

08 July 2024 7,869 1 View

Hi,I have been trying to transfect MDA MB 231 cell lines since a while, I tried with lipofectamine LTX as well as lipofectamine 3000.Please suggest?

Hi,I have been trying to transfect MDA MB 231 cell lines since a while, I tried with lipofectamine LTX as well as lipofectamine 3000.Please suggest how to proceed with this cell line and what is...

02 June 2024 1,572 0 View

What is full wave simulation in antenaa ?

What is full wave simulation in antenna ? How to simulate in CST Studio software? Can anybody explain.

20 May 2024 5,224 3 View

Can anyone explain The overall size of the proposed antenna is only 1.34λ0 × 1.32λ0 × 0.014λ0.?

Can anyone explain The overall size of the proposed antenna is only 1.34λ0 × 1.32λ0 × 0.014λ0.? What does it Mean?

20 May 2024 9,177 4 View

How to add reflector in microstrip patch antenna. ?

I want add a reflector in microstrip antenna in CST studio ? can any one suggest the procedure?

14 May 2024 3,899 2 View

I have given two port for excitation in antenna design port 1 and port 2. How to plot Realized gain vs Frequency for individual port?

Can anyone explain how to plot Realized gain vs Frequency for individual port? How to plot directivity vs d/λ?

03 May 2024 4,380 3 View

What is open-circuit stepped-impedance resonators (OCSIRs)? why it is used in antenna design?

what is open-circuit stepped-impedance resonators (OCSIRs)? why it is used in antenna design?

26 April 2024 7,583 1 View

I did a antenna design with two ports after simulation S-parameter only S11, S21 coming.? Any reason behind this error ?

kindly Share reason behind this error why only two S-parameter are coming?

26 April 2024 1,782 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View