I am using a retroviral system to transduce primary cells. We already have a working system in the lab using an MMLV-based vector together with the platE packaging cell line from Cell biolabs.
We will soon receive an MSCV vector (MSCV Luciferase PGK-hygro) from Addgene and I was wondering wether I need a different packaging cell line to produce those viruses or if I can keep on using platE cells.
Hello Ali, thank you for your answer. I have very little knowledge in virology since this is not my field. I'll try to explain my problem more clearly.
I am talking about a retroviral expression system. Basically I have a cell line (here the platE cell line) which expresses the retroviral genes gag-pol and Env from the Moloney Murine Leukemia Virus (MMLV).
I transfect this cell line with a vector containing a transgene flanked by LTR sequences which allow packaging of my gene of interest into retroviral particles. This leads to the production of replication-incompetent retroviral particles which are useful for introducing genes in hard-to-transfect cell types.
The problem here is that the LTR sequence comes from a different virus (MSCV Murine Stem Cell Virus) from the gag-pol env. Since both are retroviruses, I think it could work but I'd like to hear if someone has tried it before.
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