I have a urea quantification kit that employs urease to change urea to ammonia and then quantify ammonia based on color intensity. Can Urease do the same with the isourea derivative attached?
urea quantification kit that employs urease to change urea to ammonia and then quantify ammonia based on color intensity. For synthesis of urease in H.pylori the accessory genes of ureI, ureE, ureF,ureG, and ureH must be expressed.
UV-method for the determination of Urea and Ammonia in foodstuffs, beverages and other materials
Principle: (urease) (1) Urea + H2O → 2NH3 + CO2
(microbial glutamate dehydrogenase) (2) 2-Oxoglutarate + NADPH + NH4+ → L-glutamic acid + NADP+ + H2O
Kit size: * 50 assays of each
* The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Urea is hydrolyzed in the presence of water and urease to produce ammonia and carbon dioxide. The ammonia produced in the first reaction combines with α-oxoglutarate and NADH in the presence of glutamate-dehydrogenase to yield glutamate and NAD+. The change in absorbance at 340 nm due to the disappearance of NADH is directly proportional to the BUN concentration in the sample and is measured using a bichromatic (340, 383nm) rate technique. UREASE (Amidohydrolase, EC 3.5.1.5) is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. (NH2)2)CO + H2)O + CO2) + 2NH3) Many gastrointestinal or urinary tract pathogens produce urease. Thus its activity is a useful diagnostic parameter for the presence of pathogens such as Helicobacter pylori. Urease is found in bacteria, yeast, and higher plants.
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