How could we determine successful probe binding by a method other than PCR?

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How long could cells be cultured in 96 well plates for?

Would it be OK to culture cells in 96 well plates for over 72 hours? We need to run an experiment which involves cell seeding then treatment after 12 hours. The cells should then be incubated with...

10 November 2019 8,946 3 View

Do coagulation factors interact with cells in culture?

We are supplementing RPMI meida with human plasma instead of serum for culture of human breast cancer cells. When PRMI is supplemented with 10% human plasma, the media looks fine, however once...

07 August 2018 4,435 10 View

Why are my primers misbehaving?

We have been using a set of primers for sometime now for HCV RNA detection using conventional PCR and qPCR. All was fine; Cts for HCV positive samples, no Cts for negative samples and when water...

06 July 2018 2,833 4 View

Why is plasma/serum used in qPCR based detection and not whole blood?

Since several references indicate the presence of HCV in blood cells and that RNA extraction from whole blood samples leads to higher amounts of viral RNA. So why is plasma/serum routinely used...

06 July 2018 6,860 3 View

In patients receiving antiviral drugs, is free viral RNA/DNA from dead virions released in serum?

In patients receiving antiviral drugs, is free viral RNA/DNA from dead virions released in serum? If so will this affect the accuracy (in terms of therapeutic outcome) of qPCR based diagnostic tests?

06 July 2018 6,233 5 View

How to get rid of contamination by PCR product?

We are running qPCR for the quantification of HCV RNA. For the past two weeks, we seem to get amplification curves and Cts with: 1. Negative samples 2. Water + Mastermix + primers+ probe 3....

02 March 2018 7,008 6 View

Can I use a urea assay kit to quantify an isourea derivative?

I have a urea quantification kit that employs urease to change urea to ammonia and then quantify ammonia based on color intensity. Can Urease do the same with the isourea derivative attached?

01 February 2018 6,786 2 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View