How could we determine successful probe binding by a method other than PCR?

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How long could cells be cultured in 96 well plates for?

Would it be OK to culture cells in 96 well plates for over 72 hours? We need to run an experiment which involves cell seeding then treatment after 12 hours. The cells should then be incubated with...

10 November 2019 8,723 3 View

Do coagulation factors interact with cells in culture?

We are supplementing RPMI meida with human plasma instead of serum for culture of human breast cancer cells. When PRMI is supplemented with 10% human plasma, the media looks fine, however once...

07 August 2018 4,295 10 View

Why are my primers misbehaving?

We have been using a set of primers for sometime now for HCV RNA detection using conventional PCR and qPCR. All was fine; Cts for HCV positive samples, no Cts for negative samples and when water...

06 July 2018 2,694 4 View

In patients receiving antiviral drugs, is free viral RNA/DNA from dead virions released in serum?

In patients receiving antiviral drugs, is free viral RNA/DNA from dead virions released in serum? If so will this affect the accuracy (in terms of therapeutic outcome) of qPCR based diagnostic tests?

06 July 2018 6,092 5 View

Why is plasma/serum used in qPCR based detection and not whole blood?

Since several references indicate the presence of HCV in blood cells and that RNA extraction from whole blood samples leads to higher amounts of viral RNA. So why is plasma/serum routinely used...

06 July 2018 6,683 3 View

How to get rid of contamination by PCR product?

We are running qPCR for the quantification of HCV RNA. For the past two weeks, we seem to get amplification curves and Cts with: 1. Negative samples 2. Water + Mastermix + primers+ probe 3....

02 March 2018 6,849 6 View

Can I use a urea assay kit to quantify an isourea derivative?

I have a urea quantification kit that employs urease to change urea to ammonia and then quantify ammonia based on color intensity. Can Urease do the same with the isourea derivative attached?

01 February 2018 6,628 2 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein?

Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...

01 March 2021 8,747 4 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View