Drop plate method doesn't work good with my strains. Can I replace the drop plate method for assessing the survival of E. coli cells under heat shock conditions by heating the cell culture in the presence of the sypro orange dye?
Dear colleagues. I'm studying ORR on carbon materials, including graphene oxide. I'm new in this research direction and have questions: what are the compositions of the most suitable electrolytes...
28 August 2023 8,748 2 View
Instead of Lewis approximation I would like to use Land Weber approximation for calculation of the Swath motion kinematic characteristics. But for accurately description, a have not enough material.
01 May 2019 8,062 0 View
Dear colleagues, We need to extract lipids from sunflower seeds for UPLC-MS. Which protocol is better to take? Thank you!
22 November 2016 4,003 4 View
I need some data and information about status of minority languages in Canada (in provinces) and the USA (stats) for comparing with the regions in Russia.
10 June 2015 6,158 9 View
need to review articles on care giving in dementia and challenges faced
12 August 2024 6,424 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View