Can i sterilize Triss-HCl buffer in autoclave?

Rasha El-Mehy @Rasha_El-Mehy

06 June 2016 0 4K Report

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Science method

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Mode of action of mycorrhiza fungi and yeast extract to reduce rot root diseases and broomrape control in Faba bean fields?

Mycorrhiza fungi and yeast extract as biological control agents can reduce rot root diseases and broomrape of faba bean

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What are the cons of conventional agriculture versus sustainable agriculture?

sustinabilty of agriculture

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What are the different mechanisms used by microorganisms to produce nano-sized metal particles?

If you allow, I'm need someone explain this simply

02 March 2016 1,206 4 View

Tell me about the advanced methods of microbial enzymes determination

please answer quickly

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How to avoid RBCs agglutination on staining RBCs for flowcytometry?

Hi everybody. I have a very important question. How to avoid RBCs agglutination on adding the monoclonal antibody to RBCs? On testing my sample, I've diluted the EDTA whole blood 1:200 (RBCs count...

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What is the technique (step by step) for staining RBCs for markers: CD55 & CD59?

what is best used for gating? Glycophorin or something else?

09 October 2011 8,737 3 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

What should be the sample container for the hydrothermal reaction in a microwave reactor at 180 °C for 10 min at the heating rate of 5 °C per minute?

Suggest one of them 1. Teflon-lined stainless steel autoclave: 2. Alumina (Al2O3) ceramic container

30 July 2024 7,326 1 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View

I want to buy Hydrothermal Synthesis Autoclave from any European company. Can anyone suggest any company inside Europe?

Thanks

22 July 2024 1,143 3 View

How can EDTA be removed from already extracted DNA samples?

Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...

13 July 2024 1,675 3 View