We have -80 freezer failure and many of our glycerol stocks are dead. I want to rescue the plasmid from these stocks so we can transform new bacteria. Can you please share protocol to revive plasmid from dead bacterial glycerol stock?
I want to know how we can find "call for papers" in a special issue? Does it increases chances of quick publication and acceptance of research paper?
09 February 2021 3,748 5 View
Hi everyone, I am looking for fluorescence expression vectors for expression in gram-positive bacteria present in the skin microbiome. I need to tag them with a fluorescence marker and observe...
06 February 2021 2,566 4 View
Hello Everyone! I have prepared liposomal formulation using Rotary Evaporator method followed by dialyzed the liposomal Suspension in Phosphate buffer to remove the un-encapsulated drug. After...
25 January 2021 7,752 4 View
I need to preserve broth culture of wet fungus spore for my further research. Can any one suggest me any solution.
18 January 2021 4,756 2 View
I have computed line Wise IMF from the fringe data using empirical mode decomposition (EMD). For each line Segment I have more than five IMF sets but i am not able to understand that how to...
10 January 2021 5,036 1 View
Hello everyone This is regarding finding elastic and transport properties of a material using the above mentioned softwares. Right now I am using Quantum Espresso to find out the structural,...
28 December 2020 6,767 1 View
I want to exract genomic DNA from skin and gills of fish.
25 December 2020 6,118 3 View
Similar to the Hazen formula for estimating permeability from the gradation curve of soil, can we identify the angle of shearing resistance of silty sand and sandy silt type of soil?
03 December 2020 6,747 3 View
I am working this area, please inform some web resource that will help me.
14 October 2020 9,107 7 View
I am working on environmental and financial performance analysis of selected companies in India while reading several research papers i observed that environmental performance have been measured...
13 October 2020 527 11 View
We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...
02 March 2021 3,713 2 View
I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...
01 March 2021 9,525 4 View
I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...
28 February 2021 5,415 1 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View
One main reason for failure of most ERP implementations is the misfit between business requirents and ERP system business process be reduced. How can the misfit between business requirents and ERP...
22 February 2021 5,709 5 View
I need to take IF images with DAPI on a confocal but I need to use a glycerol fixation. Has anyone done this method before, if so how was it done? Thank you.
21 February 2021 3,081 3 View
Hi everyone, This is my first time of attempting to run survival analysis. I have a data on patients with End-stage Kidney Diseases (ESKD). I would want to run survival probability by treatment...
16 February 2021 2,647 3 View
Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...
16 February 2021 3,348 1 View
11 February 2021 3,524 2 View
Am a master student .I need an information about this case to write my thesis?
09 February 2021 9,706 2 View