We have -80 freezer failure and many of our glycerol stocks are dead. I want to rescue the plasmid from these stocks so we can transform new bacteria. Can you please share protocol to revive plasmid from dead bacterial glycerol stock?
Machine learning (ML) has shown great potential in predicting the compressive strength of concrete, an important property for structural engineering. However, its practical application comes with...
03 August 2024 2,546 2 View
here is scf file &CONTROL title = 'cal' calculation = 'scf' etot_conv_thr = 4e-6 forc_conv_thr = 1e-6 pseudo_dir='./' !tprnfor = .true. !tstress =...
29 June 2024 10,082 0 View
The title 'Behavioral Finance and Its Implications for Asset Pricing Models' has been assigned for a Master's thesis in Finance. Is this an appropriate topic? While searching, no previous works...
10 June 2024 3,906 2 View
I am doing experiments in mice model of metabolic disorder. For that I have to do quantification od 3 major short chain fatty acid namely acetate propionate and butyrate. I had earlier tried...
15 April 2024 3,284 0 View
Increasing rate of Earthquakes.
04 April 2024 6,766 3 View
I have metabolomics data from a non-targeted approach normalized with internal standards. I have calculated the p-value (t-test), VIP score, and fold change. For that, I transformed the data (each...
23 March 2024 9,663 1 View
I did LDH cytotoxicity assay on HCT116 cell line with my compounds at various concentrations. After 24,48,72h of incubation, LDH assay was performed according to manufacturer's protocol. My...
10 March 2024 8,521 0 View
Null Hypothesis: The recovery percentage of A channel is less than the recovery percentage of B channel Alternate hypothesis: The recovery percentage of A channel is not less than the recovery...
02 March 2024 4,903 4 View
The following are the results of 4 channels of recovery of NPAs H =21.402153 Chi square value= 7.814727903 , Alpha level= 0.05 P value 0.00008685210 Reject Null? Reject Null
23 February 2024 6,835 5 View
I am currently working on pin fin in microchannel. I want to calculate heat transfer and thermal boundary layer of that pin fin at different Reynolds number. Can anyone tell me how to find it in...
25 January 2024 6,611 1 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
What can be a hypothesis related to the Stock out Cost or Shortage Cost?
29 July 2024 9,259 2 View
Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...
26 July 2024 3,460 2 View
I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...
22 July 2024 6,592 3 View
Hi, I'm working on heart flow cytometry, and I use anti-mouse CCR2-BV785 (biolegend, cat150621) for staining, 20 minutes, 4 degree. But I couldn't see any CCR2+ cells. So I'm not sure if it's...
03 July 2024 3,969 4 View
Hi ResearchGate community, how are you? I need to request your help to mitigate the generation of microbubbles detected during the slide preparation of sample metaphase chromosomal spreads. I...
01 July 2024 7,694 4 View
Hi all, I am working on subroutines (UMAT and others) with Abaqus. I would need to calculate the average stress by element to eventually change its Young modulus. How should I implement that...
25 June 2024 5,205 1 View
Hi all, Second question on the same or so topic. I am working on subroutines (UMAT and others) for Abaqus to implement Tsai-Wu failure criteria for non-composite structures (I work on long...
25 June 2024 2,955 1 View
Bonjour, j'ai une question si quelqu'un a une réponse. J'ai une colonne Uptisphere Strategy 100 Å Bonding HILIC-HIT avec une taille de particules de 3 µm et une longueur de 100 mm. Je n'arrive pas...
24 June 2024 7,201 1 View
The emerging markets have suffered from a state of stagflation. I hope you know your expectations according to your experiences regarding the implications for the stock markets,,,, risks and...
19 June 2024 4,410 3 View