Can I perform Trypan Blue Dye assay for RBC?

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How to determine critical concentration for self-assembly formation??

Self-assembly is a concentration dependent phenomenon. I would like to know ways to determine the critical concentration for self-assembly to occur in peptides. Using Nile Red and measuring its...

11 December 2017 7,699 0 View

What is the reason for significant difference in the ThT fluorescence of the sample sample when recorded multiple times?

Qualitative analysis of amyloid formation can be monitored by ThT fluorescence study(Ex:440nm and Em: 485nm). Usually, at a given time, I record the Emission spectra of the sample thrice and...

07 August 2017 2,128 4 View

How to verify location of D- and L- amino acid in a peptide sequence?

I am synthesizing a peptide that contains few D- amino acid residues in between. Now that I have completed my synthesis, I would like to verify if the D-residues are incorporated in the same place...

04 May 2016 1,873 6 View

Is peptide base quantum dot a real deal?How is UV absorption pattern actually used to understand quantum confinement in case of AAs and peptides?

I was going through a paper titled 'Blue Luminescence Based on QuantumConfinement at Peptide Nanotubes' where the authors claim that they were able to develop peptide-based quantum dots from...

01 January 1970 2,968 2 View

Can deprotonation of a sample occur during ESI-MS?

I have synthesized a di-peptide using Fmoc chemistry and cleavage was done using concentrated TFA alone as no side-chain protection containing amino acid is involved in my sequence. During...

01 January 1970 5,596 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View