Jahnu Saikia

6 Questions 8 Answers 0 Followers

Questions related from Jahnu Saikia

Can I perform Trypan Blue Dye assay for RBC?

Is it a reliable technique for RBC??

11 November 2018 8,246 3 View

How to determine critical concentration for self-assembly formation??

Self-assembly is a concentration dependent phenomenon. I would like to know ways to determine the critical concentration for self-assembly to occur in peptides. Using Nile Red and measuring its...

12 December 2017 7,683 0 View

What is the reason for significant difference in the ThT fluorescence of the sample sample when recorded multiple times?

Qualitative analysis of amyloid formation can be monitored by ThT fluorescence study(Ex:440nm and Em: 485nm). Usually, at a given time, I record the Emission spectra of the sample thrice and...

08 August 2017 2,107 4 View

How to verify location of D- and L- amino acid in a peptide sequence?

I am synthesizing a peptide that contains few D- amino acid residues in between. Now that I have completed my synthesis, I would like to verify if the D-residues are incorporated in the same place...

05 May 2016 1,850 6 View

Can deprotonation of a sample occur during ESI-MS?

I have synthesized a di-peptide using Fmoc chemistry and cleavage was done using concentrated TFA alone as no side-chain protection containing amino acid is involved in my sequence. During...

01 January 1970 5,569 3 View

Is peptide base quantum dot a real deal?How is UV absorption pattern actually used to understand quantum confinement in case of AAs and peptides?

I was going through a paper titled 'Blue Luminescence Based on QuantumConfinement at Peptide Nanotubes' where the authors claim that they were able to develop peptide-based quantum dots from...

01 January 1970 2,953 2 View