Dear All,
Is it advisable to study the the telomere length and telomerase activity from stored whole blood for over 6 months? Please advise appropriate method of storing whole blood for this purpose. We are planning to conduct this study in some extreme climatic conditions requiring us to store the sample for upto a years period.
I kindly request all your inputs for this. Thanks very much
If a cell is still intact during the storage. It is should be possible to measure telomere length, after all, telomere is part of the DNA. Since mature red blood cells have no nuclei, you are actually measuring the telomeres in PBMCs. Harvesting PBMC and store them frozen in -20C may work.
you can store whole blood in CBC tubes frozen -80C
blood immediately transferred into tubes containing sterile EDTA solution (to a final concentration of 1.6 mg EDTA/ml of blood). keep in freezer Storage temperature − 80 °C for telomere length keep in mind all samples at the same temperature and nearly same freezing duration to avoid changes of telomere length by different freezing conditions
look at these papers
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581490/
http://www.biologicalproceduresonline.com/content/13/1/3
telomerase activity by PCR needs cell separation and rapid assay as it activity is lost as time passes if you need to keep samples you need special tubes I will to find the paper for you
Thanks very much for taking your time to answer the questions.
I also see these articles and I am agree with the suggestion by Eman Shov.
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