Bio-Rad IQ5 Calibration

I would like to do PCR array on IQ5 instrument. This machine has previously done calibration files. Do I need to do calibration again?

07 August 2017 5,564 1 View

Statistical Analysis in Western Blot

May I know which statistical analysis is suitable in western blot for 3 biological replicates? Using arithmetic means or can I use geometric mean?

06 July 2017 4,005 0 View

Protein Pellet Resuspension with how much DTT?

I have extracted my protein using isopropanol from Tri-reagent LS extracted cell culture. I found the pellet is difficult to dissolve in 1% SDS. I have read through some articles saying that...

31 December 2016 2,722 3 View

How can I analyse gene expression time course study by qPCR?

I am doing time course study for gene expression in cell culture. I am doing 0, 1, 2, 4, 8, 16, 24 hours. Each time point I have treated and untreated (control). I should normalised my individual...

11 December 2015 4,659 7 View

Do gene expression real time RT-PCR study have a big biological replicate differences?

I am doing real time RT-PCR of human cell line. May I know if the gene expression in different biological replicates will be different or almost the same?

10 November 2015 9,494 3 View

How do I perform a SPSS one way ANOVA test with post hoc test significant value?

I am doing on cell culture and I have done 3 biological replicates (n=3) to get my data. I treated my cells with cytokines with five different doses and is compared to control (untreated). I get...

04 May 2015 6,271 9 View

How can I combine 3 biological replicates qPCR result (in 3 diferent plates) using CFX manager Gene Study?

I have done my 3 plates for 3 biological replicates for my control and the samples. May I know how to combine all these 3 results into one? I know this can be done through CFX manager Gene Study...

04 May 2015 6,651 2 View

How can I narrow broad melt peak in qRT-PCR?

I am doing 1 step qRT-PCR using quantifast SYBR RT-PCR and using the recommended protocol by Qiagen. I get pretty narrow peak for positive control (HepG2) but not really for THP-1 induced...

01 February 2015 4,493 2 View

Any advice on Primer Design?

I have designed a set of primers with the amplicon length of 137bp, I have done my qRT-PCR but it is not specific . I have done the sequencing but the result is only showing the 23bp which is the...

11 December 2014 6,093 10 View

Will the secondary structure of PCR products affect the mobility in agarose gel electrophoresis?

08 September 2014 9,528 7 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View