Statistical Analysis in Western Blot

May I know which statistical analysis is suitable in western blot for 3 biological replicates? Using arithmetic means or can I use geometric mean?

06 July 2017 4,005 0 View

Protein Pellet Resuspension with how much DTT?

I have extracted my protein using isopropanol from Tri-reagent LS extracted cell culture. I found the pellet is difficult to dissolve in 1% SDS. I have read through some articles saying that...

31 December 2016 2,722 3 View

How can I analyse gene expression time course study by qPCR?

I am doing time course study for gene expression in cell culture. I am doing 0, 1, 2, 4, 8, 16, 24 hours. Each time point I have treated and untreated (control). I should normalised my individual...

11 December 2015 4,659 7 View

Do gene expression real time RT-PCR study have a big biological replicate differences?

I am doing real time RT-PCR of human cell line. May I know if the gene expression in different biological replicates will be different or almost the same?

10 November 2015 9,494 3 View

How do I perform a SPSS one way ANOVA test with post hoc test significant value?

I am doing on cell culture and I have done 3 biological replicates (n=3) to get my data. I treated my cells with cytokines with five different doses and is compared to control (untreated). I get...

04 May 2015 6,271 9 View

How can I combine 3 biological replicates qPCR result (in 3 diferent plates) using CFX manager Gene Study?

I have done my 3 plates for 3 biological replicates for my control and the samples. May I know how to combine all these 3 results into one? I know this can be done through CFX manager Gene Study...

04 May 2015 6,651 2 View

How can I narrow broad melt peak in qRT-PCR?

I am doing 1 step qRT-PCR using quantifast SYBR RT-PCR and using the recommended protocol by Qiagen. I get pretty narrow peak for positive control (HepG2) but not really for THP-1 induced...

01 February 2015 4,493 2 View

Any advice on Primer Design?

I have designed a set of primers with the amplicon length of 137bp, I have done my qRT-PCR but it is not specific . I have done the sequencing but the result is only showing the 23bp which is the...

11 December 2014 6,093 10 View

Can I optimize dose and time in different cell lines?

I am doing dose and time response in animal cell culture. Do I have to optimize the dose and time in one particular cell line first, then use the optimized dose for another 2 cell lines? Or, do I...

10 November 2014 5,532 2 View

Will the secondary structure of PCR products affect the mobility in agarose gel electrophoresis?

08 September 2014 9,528 7 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View