Hi iPSC users,
I've just joined a new lab and am doing iPSC culture for the first time.
The culture is pretty well set up, no problems, and all the differentiation protocols are working well.
Most of the culture conditions we use require feeding (half medium change) every 2 days.
Now, I live a 2.5h return journey away, so changing medium on the weekend is a very annoying hassle for me. But at the same time I don't want to ask someone every single weekend.
I am very tempted to try if I can feed mon-wed-fri (with an extra few 100ul to account for evaporation on Friday).
Is there any scientific reason why that should not work? Is there a reagent in the media that will be used up after 2 days, or is the feeding just because of evaporation?
I realise this will differ between cell types upon differentiation, but let's say it's just standard fibroblast-derived iPSCs.
Edit: They are in Essential-8 flex medium. But nobody here leaves them longer than every other day feeding.
depends on medium which you are going to maintain. There are medium available where you can take week-end off..flex-e6 and e8...please check.
If you chose to be a good scientist as a career, do what experiment needs not what is your convenience. There are lot of pseudo scientists in the field. It is good to come on weekend, and take time off on Monday, you will have more confidence in your results. Shorts cuts does not make good scientist.
Hi Laura-Nadine,
Yes, you can - that's what the Flex media were designed to allow. Check out the link below, the data sheet gives a suggested feeding schedule that allows weekend-free feeding.
https://www.thermofisher.com/document-connect/document-connect.html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS-Assets%2FLSG%2Fmanuals%2FEssential_8_Flex_Medium_UG.pdf&title=VXNlciBHdWlkZTogRXNzZW50aWFsIDggRmxleCBNZWRpdW0=
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