Laura-Nadine Schuhmacher

4 Questions 6 Answers 0 Followers

Questions related from Laura-Nadine Schuhmacher

Can I leave iPSCs over the weekend without feeding?

Hi iPSC users, I've just joined a new lab and am doing iPSC culture for the first time. The culture is pretty well set up, no problems, and all the differentiation protocols are working...

06 March 2019 8,533 3 View

QPCR primers with efficiency outside 80-100%: can they still be used?

Hi there, I have spend a few weeks now setting up a qPCR assay in the lab. I hope my question is straight forward without going into too much detail: After reading a lot about how to test primers...

27 April 2016 4,331 4 View

Why are my adult mouse astrocytes are not attaching?

Hi, I'm trying to make an adult (!) primary astrocyte culture from mouse cortex. I am doing this with a very experienced person who has done this before in a different lab. I am using her reagents...

02 April 2015 6,591 9 View

How do I patch-clamp channel Kir4.1 in cell cultures?

Hi, I am trying to express mouse Kir4.1 (and Kir5.1) in cell culture for whole cell patch clamp electrophysiology. I have tried Chinese hamster ovary (CHO) cells, which we normally use, and TSA201...

02 April 2015 158 4 View