Bindhya Sadhanandhan

3 Questions 2 Answers 0 Followers

Questions related from Bindhya Sadhanandhan

The plasmid, after cloning, is expressing protein but while sequencing with a Backbone specific primer it is not giving a sequence read itself?

Actual size of parent plasmid where i released insert is 8.1 ie (5.8 backbone and 2.3insert size) and actual Plasmid size which i used as backbone was 8 (5kb is backbone and 3.0 is insert). I had...

22 August 2015 5,831 4 View

How an I quantify the lentivrus integration or copy number in the cells after transduction using qRT PCR ?

i have referred many protocols, it has been mentioned that standard curve has to produced with plasmids. My question is that whether plasmid has to linearised. Cant we use circular plasmids for...

08 July 2015 3,832 5 View

Are there any databases with information regarding cancer cell lines other than sanger and CCLE?

i am checking for TSG (p16,p21,PTEN,p53) Methylation, mutation status of ovarian cancer cell line like oaw42,oaw28 ,ov167,ov177

14 August 2013 2,182 2 View