Can I leave blot in block buffer for 48 hours?

02 February 2020 1 4K Report

Is it ok ti leave the blot in non fat milk for 48 hours in +4 degrees or is it better to leave it tbs-t for that extra 24hrs?

Edward Michelini

Bacterial or even fungal growth could be a concern with long storage times in NFDM at 4C. Is there any preservative present (thimerosal, azide or kathon)?

If not the TBS-T, as Sebastian wrote, could be a better choice.

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Science method

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