Nahla Fadl

4 Questions 7 Answers 0 Followers

Questions related from Nahla Fadl

Can I leave blot in block buffer for 48 hours?

Is it ok ti leave the blot in non fat milk for 48 hours in +4 degrees or is it better to leave it tbs-t for that extra 24hrs?

02 February 2020 3,976 1 View

Should the HCV Core protein fused with His-tag (without linker) be extracted in the insoluble or soluble protein fraction?

I have infected insect cells SF9 with recomb. baculovirus expressing Core.His and another with GFP. I have extracted the soluble proteins after 3 days and did western blot using anti-his,...

02 February 2020 6,258 4 View

Is it wrong to cut the vector backbone?

I am trying to remove the EGFP insert from pEGFP-N1. I cut the insert using incompatible rest. sites and religate using gap filling PCR and standard ligation reaction. But the transformation on...

05 May 2018 9,394 9 View

What is the best method for preparing culture cells for VLP visualization with TEM?

I want to see VLP assembled in culture cells before purification under EM, is there a simple method to prepare the samples?

03 March 2018 7,354 0 View