After centrifugation and removing the growth media, can I keep the bacterial cell pellet at -80 C before RNA isolation? How about the maximum duration for keeping? I do not want to put RNAlater regent.
As long as it is purified, you might be able to keep it without RNAlater or Trizol, but I would dry it first.
You should add glycerol to cell pellet.Then, you can keep it up to 3 months.
I was in HUGO conference last week and people in microbial genetics stressed that for microbiome analysis bacterial cells should be processed on the spot. They found different expression patterns if you leave them unfrozen/fixed. I always encourage processing the samples especially RNA and protein as quickly as possible. This really minimize variability.
I hope this helps
It is better to isolate RNA as soon as possible and prevent storage before isolation. storing it for few or long time will show effect on your result as RNA are very much potent to degrade.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- RNA