Amit Kumar

6 Questions 13 Answers 0 Followers

Questions related from Amit Kumar

How can I neglect heat of dilution from heat of reaction in Isothermal Titration Calorimetry?

In my ITC run (Nano ITC, TA instruments), the heat of reaction is less than heat of dilution. For e.g. the heat of reaction for 1st and 2nd titrations was 4.17uJ and 6.23uJ respectively whereas...

03 March 2015 2,055 8 View

Why purified protein band shifted to lower molecular weight in SDS-PAGE?

I am trying to purify His-tagged protein (by Ni-NTA resin) after over-expression in BL21 cells. When i run SDS-PAGE of un-induced, induced, Lysate, supernatant, pellet and purified protein...

10 October 2014 4,975 12 View

Why am I seeing secondary structural changes in molecular dynamic simulation?

There are missing residues in the crystal structure of my protein. One part of this missing residue region (30 residues length) has no template. When I modelled this protein in modeller by taking...

05 May 2014 3,794 3 View

Temperature for the annealing step in PCR?

I am having one gene whose melting temperature for forward primer is 63 and for reverse its 70. Up to what temperature should I run the annealing temperature? Until now I did a gradient of 50-60...

03 March 2013 3,219 5 View

What are the general principles of isolating particular protein from proteome?

I have isolated proteome of a bacteria and I want to isolate and purify particular proteins from them. I am not able to understand how to design a protocol for isolation and purification of them....

03 March 2013 8,697 1 View

What will happen to stock cultures if its glycerol content is high?

What should be optimum glycerol content in stock culture and up to what time can we keep it

03 March 2013 7,762 2 View